Fig. 5.
Statins promote vascular structure formation in an in vitro Matrigel assay. HUVEC were seeded on growth factor-reduced Matrigel in the presence of simvastatin (SIM, 0.1 μM), pravastatin (PRV, 0.1 μM), VEGF (100 ng/ml) or no addition (control) in the absence of serum. Parallel HUVEC cultures were infected with adenoviral vectors (50 MOI) carrying either (dnAkt) or (myrAkt) or treated with LY294002 (10μM) at the time of seeding. a, Cultures were photographed after 8 h. b, Quantitation assessment of the extent of tube formation. *P < 0.01 relative to control.