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. Author manuscript; available in PMC: 2010 Feb 25.
Published in final edited form as: Cell Metab. 2008 Feb;7(2):159–172. doi: 10.1016/j.cmet.2007.11.003

Figure 7. Akt1-Mediated Muscle Hypertrophy Promotes Fatty Acid Metabolism in Liver.

Figure 7

(A) Comparison of transcripts significantly (p < 0.05) upregulated (red) or downregulated (blue) in liver under different experimental conditions (n = 4 in each group). Left column: comparison between control mice fed a HF/HS diet (CH) and control mice fed a normal diet (CN). Comparing these experimental conditions, 635 transcripts were upregulated and 646 transcripts were downregulated by the HF/HS diet. Right column: comparison between DTG mice fed a HF/HS diet (DH) and control mice fed a HF/HS diet (CH). 861 (67%) of the 1281 transcript expression changes in liver caused by the HF/HS diet were reversed by muscle growth.

(B) Relative mRNA expression levels of PEPCK, G6Pase, PGC-1α, HNF4α, CPT1, and SCD1 as measured by qRT-PCR in livers of control mice fed a normal diet (CN), control mice fed a HF/HS diet (CH), and DTG mice fed a HF/HS diet (DH) at the 3 week time point after DOX treatment (n = 4).

(C) Akt1-mediated muscle growth (3 weeks) promotes β-oxidation of palmitic acid in liver tissue (n = 8 in each group).

(D) Serum (left) and urine (right) ketone body levels in the different experimental groups of mice (n = 9–12 in each group). MCK-rtTA single-transgenic mice were used as a control.

Results are presented as mean ± SEM. *p < 0.05; #p < 0.01.