Figure 4.

Interaction between SYP22 and VTI12.

(A) Immunoprecipitation analysis using anti-SYP22 antiserum with zig-1, zip3-1 zig-1, vps26a zig-1, and zip1 zig-1 plant extracts. Proteins were extracted from inflorescence stems of 6-week-old plants. VTI12 and SYP22 in immunoprecipitates were detected by immunoblotting using anti-VTI12 antiserum and anti-SYP22 antiserum, respectively (first and third panels from top). The total amounts of VTI12 and SYP22 are also indicated (second and fourth panels from top).

(B) The relative incorporation ratio of VTI12 into the SNARE complex containing SYP22 (immunoprecipitated VTI12/ total VTI12) was calculated based on results shown in (A). Average values of four independent experiments are presented with error bars showing sd. Values were normalized to that of zig-1 plants. Quantification of the band intensity was performed with the software Sion Image. The statistical significance against zig-1 control was tested using a Student t test (*P < 0.05).