Figure 3.

Genetic Analysis of the F2 Population from the Cross rpl4a × rpl4d.

(A) Exon-intron organization and schematic representation of the locations of the T-DNA insertions in the RPL4A and RPL4D genes. Orange, exon; purple, intron; red, untranslated regions.

(B) RPL4 transcripts detected in 2-week-old seedlings in wild-type (Col-0) and rpl4a and rpl4d mutant alleles using RT-PCR. The positions of the oligonucleotides used for the RT-PCR (F and R) are indicated with arrows in (A).

(C) Genotypes and segregation analysis of the F2 individuals from the cross rpl4a-2 × rpl4d-1 (rpl4a × rpl4d). The possible genotypic F2 combinations derived from the rpl4a × rpl4d cross are represented with green and red bars. Green bars indicate RPL4 genomic copies without T-DNA insertions. Red bars with black triangles indicate RPL4 genomic copies containing T-DNA insertions.