Figure 1.

H₂O₂-Induced NO Accumulation in Wild-Type Roots.

The roots of 7-d-old seedlings grown on agarose half-strength Murashige and Skoog (MS) plates were treated for 30 min with 100 μ M H₂O₂ ([A] to [D]) or with the H₂O₂-generating system 0.5 mM glucose 0.5 units/mL glucose oxidase ([E] to [H]). Catalase (100 units/mL; an H₂O₂ scavenger; [C] and [G]) or 500 μ M cPTIO (an NO scavenger; [D] and [H]) was included as shown. NO production was detected using 10 μ M DAF-FM DA, a fluorescent probe for NO.