Figure 5.

PHB3 Functions in ABA-Induced NO Accumulation and Stomatal Closure.

Stomates prepared from leaves with similar sizes of 3-week old wild-type and phb3-3 mutant plants were incubated for 2 h in stomate buffer and then treated with ABA.

(A) NO production in stomates treated with 10 μ M ABA was detected by DAF-FM DA (5 μ M).

(B) Relative fluorescence signals from guard cells corresponding to treatments in (A) were quantified using the ImageJ program.

(C) and (D) Apertures (C) and ratios of width/length (D) of stomates treated with 1 and 10 μ M ABA are shown. Error bars represent sd (n = 40).