Figure 7.

Single-cell nested RT-PCR of P2X₂, P2X₃, P2Y₂, and P2Y₄ receptor subunits in LS and TL bladder neurons. A, Examples of positive nested single-cell PCR amplification of P2X and P2Y mRNAs. Product length corresponded with expected size. B, The frequency of P2X and P2Y transcription in bladder neurons is summarized. The P2X₂ subunit transcript was significantly more abundant in LS than TL bladder neurons (**p < 0.01). Error bars indicate SEM. The percentage of cells expressing P2Y₂ and P2Y₄ transcripts in P2X₂-positive (C) or P2X₃-positive (D) neurons is illustrated in pie charts.