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. Author manuscript; available in PMC: 2010 Apr 15.
Published in final edited form as: Dev Biol. 2009 Jan 14;328(2):188–199. doi: 10.1016/j.ydbio.2009.01.003

Figure 8.

Figure 8

Effect of Wnt5a addition or loss on prostatic gene expression. A–C: Gene expression in newborn rat VP lobes after 18 hr organ culture as measured by real-time RT-PCR. (A) Effect of 1μg/ml Wnt5a protein on expression of multiple developmental genes. Bars represent mean ± SEM for 15 replicates. * P <0.05 BSA vs. Wnt5a protein. (B) Left: Effect of 2 μg/ml Shh protein on Wnt5a expression in the rat VP and LP after 18 h of culture. Shh increased Wnt5a expression in LP but not VP as compared to BSA controls. Bars represent the mean ± SEM for 8 samples. ** P<0.01 Shh vs. BSA. Right: Effect of 10 nM testosterone on Wnt5a expression in the rat VP and LP after 18 hr culture. Testosterone modestly suppressed Wnt5a gene expression in both lobes. Bars represent the mean ± SEM for 8 samples. ** P<0.05 -T vs +T. (C) Effect of 1μg/ml Wnt5a protein on expression of Wnt genes and WIF1 in rat VP. Bars represent mean ± SEM for 15 replicates. ** P <0.001 BSA vs. Wnt5a protein. (D) Expression of Shh, Fgf10, Wnt2b, Wnt4, Wnt5a, Wnt11 and WIF1 mRNA in wild type and Wnt5a−/− knockout mouse UGS/prostate tissues after 4 days of culture. Bars represent mean ± SEM for 6 replicates.