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. Author manuscript; available in PMC: 2010 Feb 25.
Published in final edited form as: Cell. 2009 Jul 10;138(1):129–145. doi: 10.1016/j.cell.2009.05.047

Figure 1. Primary response genes are permissive at the basal state.

Figure 1

(A) Bone marrow-derived macrophages (BMMΦs) were treated for 1, 2, or 3 hours with LPS, or 2 hours with LPS+CHX, and analyzed by RT-qPCR. (B) Expression of housekeeping (HKG) and LPS-inducible genes in unstimulated BMMΦs was analyzed by RT-qPCR. (C) BMMΦs were stimulated for 4 hours and analyzed by ChIP (H3K4me3). (D, E) BMMΦs were analyzed by ChIP (H3K4me3, H3Ac). Genes are shaded and ranked as housekeeping (HKG), CpG-rich primary (PRG-I), CpG-poor primary (PRG-II), or secondary (SRG). Graphs magnifying the lower end of the spectrum are shown to the right. (F, G) WT and MyD88/TRIF−/− BMMΦs were analyzed by ChIP (H3Ac, H3K4me3). (A–G) Data are representative of 3 or more independent experiments. Error bars represent mean+/−SEM.