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. Author manuscript; available in PMC: 2010 Feb 25.
Published in final edited form as: Cell. 2009 Jul 10;138(1):129–145. doi: 10.1016/j.cell.2009.05.047

Figure 4. Brd4 is recruited to PRG promoters following inducible acetylation of H4K5/8/12.

Figure 4

(A) BMMΦs were stimulated for 1 hour with LPS and analyzed by ChIP (Brd4). (B, C) BMMΦs were analyzed by ChIP (H3K9Ac, H4K5Ac, H4K8Ac, H4K12Ac). (D) BMMΦs were stimulated for 1 hour with LPS and analyzed by ChIP (H4K5Ac, H4K8Ac, H4K12Ac). (E) Histone peptide binding assays were performed with GST fusion proteins of bromodomain 1 or 2 of Brd4 (BD1, BD2) and either no peptide, AcH4 peptide, or unmodified H4 peptide. Reactions were analyzed by Western blotting with anti-GST. (F) BMMΦs were stimulated for 1 hour with LPS and analyzed by ChIP (p300/CBP, PCAF, GCN5). (G) Schematic of the H4 tail. (A–F) Data are representative of 3 or more independent experiments. Error bars represent mean+/−SEM.