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. Author manuscript; available in PMC: 2011 Mar 1.
Published in final edited form as: Microvasc Res. 2009 Dec 3;79(2):128–138. doi: 10.1016/j.mvr.2009.11.007

Figure 4. Effect of PKA activation on thrombin-induced EC barrier dysfunction and Rho signaling.

Figure 4

A - Endothelial monolayers were pre-treated with 6Bnz (100 μM, indicated by first arrow) and stimulated with thrombin (0.1 U/ml, indicated by second arrow). TER measurements were performed over 3 hrs. B - Site-specific phosphorylation of MYPT1 and MLC in lung EC challenged with thrombin for 5, 15, or 30 min with or without 6Bnz pretreatment was detected by western blot. C - Rho phosphorylation was determined in control and 6Bnz-treated (100 μM, 30 min) cells by immunofluorescence with phospho-Ser188-specific antibodies. D - HPAEC transfected with Rac-specific or non-specific RNA for 48 hrs were stimulated with thrombin with or without 6Bnz pretreatment. Levels of phosphorylated MLC were determined by western blot analysis.