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. Author manuscript; available in PMC: 2010 Dec 1.
Published in final edited form as: Arthritis Rheum. 2009 Dec;60(12):3784–3793. doi: 10.1002/art.24977

Figure 1. Expression of Mi-2 and NCAM in DM muscle.

Figure 1

Paraffin cross sections from a DM muscle biopsy specimen were analyzed by immunofluorescence. (A, D, and G) A low power (20 X) view of affected fascicles shows the highest expression of cytoplasmic NCAM (red) and nuclear Mi-2 (green) in perifascicular regions. (B, E, and H) A higher power (40X) view of the same region with arrows indicating fibers with centralized nuclei staining strongly for Mi-2 and NCAM. (C, F, and I) Nuclei of histologically normal appearing muscle from the middle of a fascicle from the same specimen express no NCAM and relatively low levels of Mi-2. To insure comparability, images B, C, E, F, H, and I were obtained using identical exposure settings for each channel. Images show NCAM alone (A, B, and C), Mi-2 alone (D, E, and F), and merged NCAM, Mi-2, and DAPI (G, H, I).