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. 2010 Feb 22;188(4):565–579. doi: 10.1083/jcb.200908055

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© 2010 Owald et al.

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Figure 2. — DSyd-1 localizes to central synapses. (A) In situ hybridizations show that dsyd-1 is expressed throughout the embryo’s CNS. st., stage. (B) Confocal z projection of adult Drosophila CNS. α-DSyd-1 staining colocalizes with BRP^Nc82 throughout the brain, but is absent in dsyd-1 animals (C). (D) DSyd-1 localizes opposite to postsynaptic acetylcholine receptors (Dα7^GFP) expressed in Kenyon cells at the adult MB calyx. Arrowheads in the inset panels (which show enlarged views) indicate pre- to postsynaptic alignment. Bars: (B and C) 50 µm; (D) 10 µm; (D, insets) 500 nm.