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. 2010 Feb 22;188(4):565–579. doi: 10.1083/jcb.200908055

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© 2010 Owald et al.

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Figure 9. — DSyd-1 accumulates early during AZ assembly. Confocal stacks of sequentially in vivo imaged NMJs (muscle 26), Δt = 12 h. NMJs coexpressing ^GFPDSyd-1 and BRP-short^mStraw (A), and DLiprin-α^GFP and ^mStrawDSyd-1 (B). (A) DSyd-1 preceded BRP (arrows and arrowheads) at 65% of the newly forming AZs, and BRP preceded DSyd-1 at 0%. The situation was not resolved at 35% (n = 37). (B) DLiprin-α and DSyd-1 accumulate in close temporal proximity (arrows and arrowheads): DLiprin-α preceded DSyd-1 at 26% of newly forming AZs, and DSyd-1 preceded DLiprin-α at 6%. The situation was not resolved at 68% (n = 35). Bars: (A and B) 4 µm; (A and B, insets) 500 nm.