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. 2010 Feb 22;188(4):547–563. doi: 10.1083/jcb.200908086

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© 2010 Koivusalo et al.

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Figure 3. — Effect of Na⁺ omission on macropinocytosis and pH_c. (A) Epifluorescence images of islands of A431 cells after 10 min incubation with TMR-dextran and EGF in the indicated Na⁺-free media. To clamp pH_c (bottom panel), cells were preincubated in K⁺/nigericin. Bar = 10 µm. (B) Quantification of macropinocytosis in Na⁺-free solutions. Data are means ± SE of 3–4 experiments. Data were compared with controls in Na⁺-rich medium (as in Fig. 1 B) and significance calculated using Student’s t test; ***, P < 0.001. (C) Measurement of pH_c using SNARF-5F in cells treated with EGF in the indicated buffers. Data are means ± SE of three experiments, each measuring 10 cells.