Figure 5.

Acb1 is necessary for sporulation. Wild-type, atg1Δ, atg8Δ, and acb1Δ cells were incubated in 1% potassium acetate solution at 30°C with rotation at 250 rpm. An aliquot of acb1Δ cells was incubated with 4 nM recombinant AcbA during the sporulation assay and analyzed for production of spores. 1 ml of the culture was processed as explained in Materials and methods to destroy viable cells. The spore preparation was appropriately diluted and plated on YPD plates. Plates were incubated for 2–3 d, and colonies were counted. Spore production for individual strains was expressed as the percentage of the wild-type spore count. One of two experiments is shown. Wild-type and acb1Δ cells were subjected to the sporulation assay as described in Materials and methods.