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. 2010 Mar 1;21(5):725–738. doi: 10.1091/mbc.E09-04-0268

Figure 4.

Figure 4.

Subcellular distribution of LIN-23 in early embryonic blastomeres and of LIN-23 and CDC-25.1 in adult germline. (A–E) Indirect immunostaining of LIN-23 in wild type (A–D) or lin-23(RNAi)–treated embryos (E) by using an anti-LIN-23 antibody (red panels) with corresponding DAPI counterparts (blue panels). Early two-cell embryo (A), late two-cell embryo (B), four-cell embryo (C and E), and 28-cell embryo (D). Nuclear localization of LIN-23 indicated by white arrows in the blastomere AB (B) and ABa and Abp (C). Bar, 10 μm. (F) Indirect immunostaining of LIN-23 in the adult germline of the wild-type strain N2 (green panels) with corresponding DAPI counterparts (blue panels). The position of oocyte nuclei are marked with white arrows and the turn of the gonad labeled with T. The proximal and distal arms of the gonad are labeled and both shown are from the same intact isolated gonad. Bar, 10 μm. (G–H) Indirect immunostaining of LIN-23 in the adult germline of the wild-type strain N2 (green panels) with corresponding DAPI counterparts (blue panels). The section shown is from approximately the middle of the distal arm. The nuclei are packed around the outside of the distal arm, as seen in the top focal plane (G). The core of the distal arm does not contain nuclei, but has strong LIN-23 staining (H). Bar, 10 μm. (I) Indirect immunostaining of CDC-25.1 in the adult germline of the wild-type strain N2 (green panels) with corresponding DAPI counterparts (blue panels). The position of oocyte nuclei are marked with white arrows and the turn of the gonad labeled with T. The proximal and distal arms of the gonad are labeled and are shown in the same orientation as that for F. Bar, 10 μm. (J) Indirect immunostaining of LIN-23 in the adult germline of the wild-type strain N2 (green panels) with corresponding DAPI counterparts (blue panels). The section shown is from approximately the middle of the distal arm similar to the section shown in G, but at higher magnification. Bar, 10 μm. (K) Indirect immunostaining of CDC-25.1 in the adult germline of the wild-type strain N2 (green panels) with corresponding DAPI counterparts (blue panels). The section shown is from approximately the middle of the distal arm similar to the section shown in G, but at higher magnification. Bar, 10 μm.