Table 2.
Straina | Distance migrated (μm)/30 s |
Nuclear oscillationsb |
Nuclear bypassing |
||||||
---|---|---|---|---|---|---|---|---|---|
Avg. | max | n | pc value | Forward movement | Backward movement | Events | Total nuclei | % | |
Wild-type | 0.51 | 2.82 | 1264 | 101 | 41 | 15 | 48 | 31 | |
spc72Δ | 0.35 | 1.39 | 1177 | <0.001 | 48 | 0 | 4 | 42 | 10 |
(2.12) | (8.91) | (82)d | <0.001 | (8)e | |||||
nud1Δ | 0.45 | 2.3 | 861 | <0.01 | 81 | 28 | 8 | 36 | 22 |
cnm67Δ | 0.56 | 3.11 | 770 | <0.05 | 138 | 28 | 17 | 25 | 68 |
stu2Δ | 0.36 | 1.23 | 240 | <0.001 | 9 | 0 | 0 | 32 | 0 |
a Background AgH4-GFP.
b The movements of the five most apical nuclei in four different hyphae (1 hypha for stu2Δ) were followed >40 or >30-s time intervals. Forward or backward displacements ≥0.75 μm (approximately half the diameter of a nucleus) within 30 s were scored.
c Statistical significance between mutant and wild-type values was calculated using an unpaired Students t test, and the resulting p value is shown. We also compared different mutant data sets with each other and all average values are highly statistically significant except for the slight difference in the average values from Agspc72Δ and Agstu2Δ mutants (p > 0.05).
d A subpopulation of nuclei in Agspc72Δ mutants undergo fast, long-range bypassing (see Figure 2). The numbers in brackets concern these nuclei, which were excluded when the average of parameters was determined.
e Each bypassed nucleus was counted.