Figure 4.

High-resolution ChIP-on-chip analysis of β-actin binding to chromosome 2. Cross-linked and sonicated chromatin was prepared from HL-60 cells with or without PMA treatment for 48 h. ChIP experiments were performed, and then the precipitated DNAs prepared from a β-actin antibody and a nonspecific IgG as well as total input DNA were used to prepare amplicons by LM-PCR. High-density HG18_RefSeq_promoter arrays were hybridized with the amplicons. (A) Representative profile of the β-actin complex along the chromosome 2 was shown. The logarithmic ratio (log₂R) means hybridization intensities between β-actin or IgG enriched DNA and input DNA. (B) A close-up view of ChIP-on-chip hybridization signals for β-actin and nonspecific IgG binding around the 5′-end of SLC11A1 gene.