Figure 7.

Effects of β-actin knockdown on the expression of six target genes. HL-60 cells were transfected with either control (−) or β-actin (+) siRNA and were then treated with PMA as described in Figure 7. The expression levels of six target genes SLC11A1, TCF23, PEX13, ZNF638, NCOA1, and ANXA4 and one nontarget gene, SCG2, were measured by real-time PCR. Experiments were conducted in quadruplicate and normalized to GAPDH mRNA level (data shown are mean ± SEM, n = 4, unpaired t test, **p < 0.001 compared with mRNA level in β-actin siRNA-treated cells at the corresponding time point of PMA treatment).