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. 2010 Feb 1;5:4. doi: 10.1186/1749-8104-5-4

Figure 4.

Figure 4

unf impacts all mushroom body neurons early during development. OK107-GAL4 was used to drive expression of the UAS-mCD8GFP transgene in all MB neurons and their axons (white). (A) In UAS-mCD8GFP;;OK107-GAL4 late third instar larvae controls, γ neurons project larval-specific axons medially and dorsally. (B) In unfX1 mutant late third instar larvae, γ neurons project medially only; dorsal axons are missing (star). (C) Medial and dorsal γ axons are present in unfX1 rescue third instar larvae. (D) In UAS-mCD8GFP;;OK107-GAL4 control pupae at 18 hours APF, γ axons have been pruned back to the peduncle and only axon fragments are visible; some β' and α' axons project medially and dorsally, respectively. (E) In unfX1 mutant pupae at 18 hours APF, γ axons have been pruned with some fragments visible (arrow); β' and α' axons are completely missing (stars). (F) All MB lobes are visible in UAS-mCD8GFP;;OK107-GAL4 control pupae at 48 hours APF. (G) All MB lobes are absent in unfX1 mutant pupae at 48 hours APF (stars). Scale bars = 10 μm.