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. 2010 Feb 1;5:4. doi: 10.1186/1749-8104-5-4

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Copyright ©2010 Bates et al; licensee BioMed Central Ltd.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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201Y-GAL4 and c739-GAL4 driven GFP expression is unf-dependent. The UAS-mCD8GFP reporter was used with three enhancer trap constructs to visualize MB neurons (Kenyon cells (KCs), green). Fluorescently labeled phalloidin (red) was used to visualize actin-rich structures, including the dendritic fields (calyces) of the MBs and the protocerebral bridge (Pb) that lies in the same plane. (A, B) The c747-GAL4 transgene drives the expression of GFP in MB neurons in UAS-mCD8GFP/c747-GAL4 controls and in unf^X1,UAS-mCD8GFP/Df2426,c747-GAL4 mutants. (C, D) The 201Y-GAL4 transgene drives the expression of GFP in a subset of the MB neurons in UAS-mCD8GFP/201Y-GAL4 controls (C), but unf^X1,UAS-mCD8GFP/Df2426,201Y-GAL4 mutants do not express 201Y-GAL4-driven GFP in MB neurons (D). (E, F) UAS-mCD8GFP/c739-GAL4 controls express GFP in MB neurons (E). GFP expression is greatly reduced in unf^X1,UAS-mCD8GFP/Df2426,c739-GAL4 mutants (F). The calyx and Pb are positively labeled with phalloidin in all mutants and controls. Scale bars = 10 μm.