Table 1.
Mushroom body phenotypes in unf mutants and rescue animals.
| Row | Genotype | All lobes present adult/larvae (%) | All lobes missing adult/larvae (%) | Dorsal lobes missing adult/larvae (%) | Missing a dorsal or medial lobe adult/larvae (%) | n adult/larvae |
|---|---|---|---|---|---|---|
| Controls | ||||||
| 1 | w1118 | 100 | 0 | 0 | 0 | 10 |
| 2 | unfX1/+ | 100/100 | 0/0 | 0/0 | 0/0 | 12/8 |
| 3 | unfZ0001/+ | 100/100 | 0/0 | 0/0 | 0/0 | 7/15 |
| 4 | Df/CyOGFP | 100/100 | 0/0 | 0/0 | 0/0 | 8/8 |
| 5 | UAS-GFP;;OK107 | 100 | 0 | 0 | 0 | 10 |
| 6 | unfX1, UAS-GFP/CyOGFP;;OK107 | 100 | 0 | 0 | 0 | 15 |
| 7 | unfZ0001, UAS-GFP/CyOGFP;;OK107 | 90 | 0 | 10 | 0 | 10 |
| unf mutants and gene knock down | ||||||
| 8 | unfX1,UAS-GFP/Df;;OK107 | 0/0 | 100*/0 | 0/90* | 0/10 | 15/11 |
| 9 | unfX1,UAS-GFP/unfX1;;OK107 | 0 | 100* | 0 | 0 | 7 |
| 10 | unfZ0001/Df, UAS-GFP;;OK107 | 0/0 | 0/0 | 100*/100* | 0/0 | 13/6 |
| 11 | unfZ0001/unfZ0001,UAS-GFP;;OK107 | 37.5 | 0 | 37.5 | 25 | 8 |
| 12 | unfMB05909/Df, UAS-GFP;;OK107 | 100 | 0 | 0 | 0 | 14 |
| 13 | +/Df;UAS-unfRNAi;OK107 | 50 | 0 | 40 | 10 | 10 |
| 14 | unfX1,UAS-GFP/unfZ0001;;OK107 | 0 | 13 | 47 | 40 | 15 |
| unf transgenic rescues | ||||||
| 15a | unfX1,UAS-GFP/Df;UAS-unfrsqF/+;OK107 | 100*/83* | 0/0 | 0/0 | 0/17 | 11/6 |
| 16b | unfZ0001/Df, UAS-GFP/UAS-unfrsqF/+;OK107 | 100* | 0 | 0 | 0 | 8 |
| 17c | unfX1,UAS-GFP/Df;UAS-unfrsqC/+;OK107 | 86* | 0 | 0 | 14 | 7 |
| unf rescue controls | ||||||
| 18a | unfX1,UAS-GFP/Df;TM3Sb/+;OK107 | 0 | 100 | 0 | 0 | 6 |
| 19a | unfX1,UAS-GFP/CyO;UAS-unfrsqF/+;OK107 | 100 | 0 | 0 | 0 | 9 |
| 20a | unfX1,UAS-GFP/CyO;TM3Sb/+;OK107 | 100 | 0 | 0 | 0 | 4 |
| 21a | Df/CyOGFP;UAS-unfrsqF/+;OK107 | 100 | 0 | 0 | 0 | 5 |
| 22a | Df/CyOGFP;TM3Sb/+;OK107 | 100 | 0 | 0 | 0 | 7 |
| 23b | unfZ0001/Df, UAS-GFP;TM3Sb/+;OK107 | 0 | 0 | 100 | 0 | 7 |
| 24c | unfX1,UAS-GFP/Df;TM3Sb/+;OK107 | 0 | 100 | 0 | 0 | 9 |
| 25 | unfX1,UAS-GFP/Df;UAS-lacZ/+;OK107 | 0 | 100 | 0 | 0 | 9 |
| 26 | UAS-GFP;UAS-unfrsqF/+;OK107 | 100 | 0 | 0 | 0 | 5 |
Data are presented as percentages of whole brains that exhibit the phenotype. Control and mutant animals are 0- to 2-day adults, whereas rescue and rescue control animals are 72- to 96-hour pupae. All larvae are third instars. Single entries are adult or late pupae. Rescues and the corresponding control siblings are noted by matching subscript letters in rows. Asterisks indicate P-values of < 0.01 from the Fisher exact test. Abbreviations: Df, Df(2R)ED2426; UAS-GFP, UAS-mCD8GFP; OK107, OK107-GAL4.