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. 2010 Jan 14;11:4. doi: 10.1186/1471-2202-11-4

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Copyright ©2010 Tognoli et al; licensee BioMed Central Ltd.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Stress does not alter the ratio of pro/matBDNF in the seabass liver. A) A representative Western blot of proBDNF and matBDNF expression in the seabass liver. Alpha-tubulin (MW 55 KDa) is used for normalization. Human recombinant proBDNF and matBDNF shows reactivity of the anti-BDNF antibody. The bands corresponding to the seabass proBDNF and matBDNF are indicated on the right side. B) Mean percentage of proBDNF vs. total BDNF (totalBDNF = proBDNF+matBDNF) of 10 control and 10 stressed animals. Error bars represent SE. No significant difference between control and stressed animals was found. C) Scatter-plot showing the large overlap of the percentage of proBDNF on total BDNF in individual animals from the control and the stressed populations. The F values, obtained by One way Anova analysis, for proBDNF and mature BDNF were 4.154 and 0.055, respectively.