Figure 3. The loss of C-NHEJ greatly reduces end joining.

The indicated cell lines were transfected with HindIII- (Top panels) or I-SceI- (Bottom panels) linearized pEGFP-Pem1-Ad2 plasmid together with a pCherry plasmid. The cells were analyzed by FACS 24 hr post transfection. The number of cells that were doubly EGFP (horizontal) and pCherry (vertical) positive versus the number that were pCherry positive was determined. For a given experiment the data are shown as percent repair in the upper right-hand corner of each plot.