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. 2010 Feb 26;6(2):e1000857. doi: 10.1371/journal.pgen.1000857

Figure 6. Wolbachia-dependent lifespan extension of dilp2–3,5 mutants is correlated with xenobiotic resistance.

Figure 6

Wolbachia affects longevity (A) and xenobiotic resistance (E) of dilp2–3,5 mutants, but not fecundity (B), starvation (C) or oxidative stress resistance (D). (A) Median and maximum lifespan of wDah;dilp2–3,5 females (83/92,5 days) compared to wDah (64,5/76 days) control females is increased by 29% and 22%, respectively. ** p<0.001, log rank test. In contrast, wDahT;dilp2–3,5 (62,5/81 days) only show a small increase in maximum, but not in median lifespan compared to wDahT (64,5/74 days) females. (B) Index of lifetime fecundity of dilp2–3,5 females is reduced to 30% but not affected by Wolbachia infection. Shown is the cumulative number of eggs laid by an average female. ** p<0.01, Wilcoxon rank sum test. (C–E) Survival of dilp2–3,5 females with and without Wolbachia on (C) 1,5% agarose, (D) 5% hydrogen peroxide and (E) DDT (275 mg/l). ** p<0.01, log rank test. (F) PCR analysis confirms the presence of Wolbachia in wDah flies and the absence in Tetracycline-treated wDahT flies. (G) Q-RT-PCR analysis shows that 4E-BP expression is not affected by Wolbachia-infection status. 4E-BP transcript level in Wolbachia- flies was normalised to the corresponding Wolbachia+ sample, which by default was set to 1.