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. 2010 Feb 26;5(2):e9356. doi: 10.1371/journal.pone.0009356

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This is an open-access article distributed under the terms of the Creative Commons Public Domain declaration which stipulates that, once placed in the public domain, this work may be freely reproduced, distributed, transmitted, modified, built upon, or otherwise used by anyone for any lawful purpose.

This is an open-access article distributed under the terms of the Creative Commons Public Domain declaration, which stipulates that, once placed in the public domain, this work may be freely reproduced, distributed, transmitted, modified, built upon, or otherwise used by anyone for any lawful purpose.

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(A) Isothermal titration calorimetry for stable mutant PAI-1 interaction with SMTB ^1–44. Base line-adjusted heats per injection of SMTB ^1–44 (10 µM) into PAI-1 (1.0 µM). (B) Molar enthalpies per injection for PAI-1 interaction with SMTB ^1–44. Filled circles, measured enthalpies; solid line, fit of experimental data to a single-site binding model. Thermodynamic parameters: ΔH = −24±1.34 kcal/mol. (C) Base line-adjusted heats per injection of SMTB ^1–44 (10 µM) into Cyr61 (1.0 µM). (D) Molar enthalpies per injection for SMTB ^1–44 interaction with Cyr61 where no heat exchange is observable. Filled circles, measured enthalpies.