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. 2010 Feb 26;5(2):e9356. doi: 10.1371/journal.pone.0009356

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This is an open-access article distributed under the terms of the Creative Commons Public Domain declaration which stipulates that, once placed in the public domain, this work may be freely reproduced, distributed, transmitted, modified, built upon, or otherwise used by anyone for any lawful purpose.

This is an open-access article distributed under the terms of the Creative Commons Public Domain declaration, which stipulates that, once placed in the public domain, this work may be freely reproduced, distributed, transmitted, modified, built upon, or otherwise used by anyone for any lawful purpose.

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Indicated concentrations of native monomeric VTNC, heat-modified monomeric VTNC, multimeric VTNC, SMTB ^1–44, or ixolaris were co-incubated with a constant concentration of Cyr61 (30 nM) on microtiter wells coated with multimeric VTNC. The binding of Cyr61 to immobilized VTNC was detected with anti-Cyr monoclonal antibody followed by alkaline phosphatase-labeled anti-mouse secondary antibody and appropriate substrate as described in Materials and Methods. Results were from quadruplicate experiments.