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. 2010 Feb 26;5(2):e9356. doi: 10.1371/journal.pone.0009356

Table 2. Kinetics of PAI-1 interactions with SMTB 1–44.

ka1 (M−1s−1) kd1 (s−1) KD (pM)
SMTB 1–44 2.312×106 0.00010 43.25

Responses were obtained by injecting stable mutant PAI-1 over immobilized SMTB 1–44 for 90 seconds at a flow rate of 30 µl/minute. Data were derived from Ka1 and Kd1 and fitted using the Langmuir (1∶1 binding) equation. Kinetic values obtained from the sensorgrams presented in Figure 3D.