Table 2. Kinetics of PAI-1 interactions with SMTB 1–44.
ka1 (M−1s−1) | kd1 (s−1) | KD (pM) | |
SMTB 1–44 | 2.312×106 | 0.00010 | 43.25 |
Responses were obtained by injecting stable mutant PAI-1 over immobilized SMTB 1–44 for 90 seconds at a flow rate of 30 µl/minute. Data were derived from Ka1 and Kd1 and fitted using the Langmuir (1∶1 binding) equation. Kinetic values obtained from the sensorgrams presented in Figure 3D.