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The purified flagellins (120 ng) were resolved either on 5% to 15% SDS (A) or non-denturing polyacrylamide gels (B) and stained with Coomassie blue. Purifed flagellin (50 ng, C) or bacterial extracts (60 µg, D) resolved on SDS-PAGE and transferred to nitrocellulose were also probed with flagellin antibody.
