Figure 6. Flagellin Mutated in TLR5 binding region fails to silence PAK-triggered inflammatory signaling pathways in HCECs.

HUCL cells were either pretreated with 50 ng/ml PAK (PF) or L94A (LF) flagellin or left untreated (C) for 24 h and then challenged with (PAK) or without (Control) live PAK for 1 h. Total cell lysates were blotted with antibodies specific for phospho-ERK, AKT, IκB-α, and P38, followed by reprobing with antibodies against corresponding proteins. The data shown are representative of 2 independent experiments.