Figure 7. Flagellin Mutated in TLR5 binding region fails to blunt PAK-induced cytokine production in HCECs.

HUCL cells were pretreated without (C) or with 50 ng/ml PAK (PF) or L94A (LF) flagellin for 24 h. After washed twice with PBS, cells were stimulated with live PAK for 4 h. Culture supernatant was analyzed for IL-6, IL-8 with ELISA or for hBD2 and LL-37 production using slot-blot. The intensity of each dots was determined by Photoshop histogram analysis and expressed mean of pixels. Data shown are representative of two independent experiments. *P<0.01 (N = 3).