Table 2. Prevalence of Hes3+ cells in adult rat central nervous system areas.

Area	Hes3+ cell#/field	SD
SVZ	9.4	4.4
Striatum	0.5	0.4
Substantia Nigra	0.9	0.8
Hippocampus	0.7	0.7
Cerebral cortex	0.4	0.2
Lateral forebrain	1.3	1.2
Aqueduct	3.7	1.3
Spinal Cord	0.4	0.3

Adult rats were perfused and 16 micrometer – thick sections were prepared from the brain and spinal cord. The sections were immunolabeled for Hes3 and the Hes3+ cell numbers were counted using a fluorescent microscope with a x40 objective. The field of view is 0.05 micrometers squared.