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. 2010 Feb 26;5(2):e9365. doi: 10.1371/journal.pone.0009365

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This is an open-access article distributed under the terms of the Creative Commons Public Domain declaration which stipulates that, once placed in the public domain, this work may be freely reproduced, distributed, transmitted, modified, built upon, or otherwise used by anyone for any lawful purpose.

This is an open-access article distributed under the terms of the Creative Commons Public Domain declaration, which stipulates that, once placed in the public domain, this work may be freely reproduced, distributed, transmitted, modified, built upon, or otherwise used by anyone for any lawful purpose.

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Representative magnetic resonance imaging and corresponding histochemical analysis of radiation injured (8 weeks after 50Gy focal irradiation) animal that received labeled CTL. (A) T2*-weighted image showed no definite low signal intensity area at the site of radiation injury (arrow) compared to contralateral hemisphere. (B) Corresponding R2* map also showed no high signal intensity area. Luxol fast blue staining revealed extensive loss of myelination at the site of radiation injury (C) compared to that at the corresponding site of contralateral normal hemisphere (D). FITC-tagged tomato lectin staining showed loss of normal blood vessels morphology with evidence of dilatation (arrows) at the corresponding site of radiation injury (demyelinated area) (E), in contrast to the vessel distribution seen in the corresponding site of the contralateral normal hemisphere (F). DAB enhanced Prussian blue staining showed no Prussian blue positive cells at the corresponding radiation injured or contralateral normal hemispheres (G and H). Luxol fast blue, lectin and Prussian blue stained tissues sections were obtained from consecutive slices.