Figure 1. Effect of DevR_N-Kan and full-length DevR co-expression on DevR regulon gene expression.

M. tb lysates were electrophoresed and subjected to immunoblot analysis using polyclonal antibodies to HspX (top panel), DevR (middle panel) and SigA (bottom panel). Lanes 1, 3, 5, 7, 9, 11 and 13 represent aerobic culture and lanes 2, 4, 6, 8, 10, 12 and 14 represent 5 days standing hypoxic cultures. Anti-HspX immunoblots were developed for longer periods to visualize HspX in Comp1 bacterial lysates. Representative blots from 2 to 4 independent cultures are shown.