Table 2. M. tb strains used in this study.

M. tb strain	Description	Expression		Reference
		DevR Aer Hyp	DevRN-Kan Aer Hyp
H37Rv	WT	+ +++	−−	[13]
Mut1	devRΔCTD, expresses DevRN-Kan protein from its native location (fusion gene created by in-frame insertion of promoterless kanamycin resistance cassette at the PpuMI site within devR gene), includes entire N-terminal signaling domain of DevR [residues 1–145], Kanr	−−	+ ++ +++	[13], this study
Mut2	Complete ΔdevR deletion	− −	− −	[12]
Comp1	M. tb Mut1 complemented with plasmid pDSDevR, Kanr, Hygr	+ ↓	+++ +++	[13]
Comp2	M. tb Mut2 complemented with pDSDevR, Kanr, Hygr	+ ↓	−−	This study
Comp3	H37Rv containing pAVDevRN-Kan, Kanr, Hygr	+ ++	+ + + +++	This study
Comp4	M. tb Comp2 containing pAVDevRN-Kan, Kanr, Hygr	+ ↓	+++ +++	This study

Aer, aerobic; Hyp, hypoxic.

+, ++ etc., relative levels of DevR and DevR_N-Kan proteins (semi- quantitative).

−, absence of DevR.

↓, decline in hypoxic level.