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. Author manuscript; available in PMC: 2010 Feb 25.
Published in final edited form as: Oncogene. 2009 Jun 15;28(33):2988–2998. doi: 10.1038/onc.2009.152

Figure 2. Endogenous MST1 and MST2 immunoprecipitate with endogenous RASSF2, and the majority of endogenous MST1 and MST2 is bound to endogenous RASSF2 in mammalian cells.

Figure 2

To investigate whether endogenous RASSF2 interacts with endogenous MST1 or MST2 we immunoprecipitated MST1, MST2 and RASSF2 from A549 cells. In immunoprecipitates of RASSF2 both MST1 and MST2 were detected (a), and RASSF2 co-immunoprecipitated with both MST1 and MST2 (b). * corresponds to IgG heavy chain. To ascertain what fraction of endogenous MST1 or MST2 is associated with endogenous RASSF2 we immunodepleted RASSF2 from A549 protein lysates and immunoblotted the RASSF2-bound and unbound fractions (c; I is input, s/n supernatant, and B bound fraction. β-actin was included to control for equal loading of input and supernatant and to control for specificity of the immunoprecipitation). Thus the majority of both MST1 and MST2 co-immunoprecipitate with RASSF2.