Figure 4.
HipHop is enriched at polytene telomeres. (A) Anti-HipHop staining on wild-type polytene chromosomes. DAPI-stained DNA in white, antibody signals in red. Telomeres are marked with * except that of the X chromosome, which was out of the picture. The band marked with ** is non-specific as it was not detected by a second anti-HipHop serum. (B, B', B'') HOAP and HipHop co-localization on a single wild-type telomere. Signals from anti-HOAP are in green (B), those from anti-HipHop in red (B') and merged signals are in yellow (B''). The extra-chromosomal signals in (B) are non-specific. (C) Genetic control of HipHop loading to polytene telomeres. Genotypes are shown on top of the pictures. DAPI-stained telomeres (marked by *) are labelled as ‘DNA' and shown in the top panels. Signals from anti-HipHop (HipHop) are shown in the bottom panels.