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. Author manuscript; available in PMC: 2010 Oct 1.
Published in final edited form as: Genesis. 2009 Oct;47(10):680–687. doi: 10.1002/dvg.20547

Figure 3. Marker analyses in e13.5 embryonic spinal cords derived from GFP-tagged floxed Hoxc8 and floxed Hoxc8->c9 alleles prior to Cre-mediated recombination.

Figure 3

(a–e) Hoxc8 mRNA expression in open-book preparations of e13.5 embryonic spinal cords. Slight changes in the rostral boundary of Hoxc8 expression domain in the ventral (red boxed areas) and intermediate (open arrows) spinal cord are observed in Hoxc8F/F-GFP (c), Hoxc8->c9F/+-GFP (d), and Hoxc8->c9F/F -GFP (e) embryos as compared to the wild-type (a) and Hoxc8F/+ -GFP (b) embryos.

(f–j) Hoxc9 mRNA expression in open-book preparations of e13.5 embryonic spinal cords. Hoxc9 rostral expression boundary (red boxed area) in the Hoxc8->c9F/F -GFP (j) embryos is placed ~1 segment caudally when compared to the rest of embryos (f–i).

(k–o) mRNA expression of an LMC marker, Raldh2, in open-book preparations of e13.5 embryonic spinal cords. ~1 segment caudal extension of Raldh2 expression is observed in the Hoxc8->c9F/F -GFP embryo (o) compared to the rest of embryos (k–n).

(p–t) mRNA expression of a motor pool marker, Pea3, in open-book preparations of e13.5 embryonic spinal cords. A caudal expansion of Pea3 expression is observed in Hoxc8->c9F/F-GFP embryos (t). Scale Bars=0.5mm.