Figure 7.

Confocal images of CHO-A1 cells incubated (5 min) with: (A) APrEA-X-BY630 (10 nM), (B) ADOEA-X-BY630 (10 nM), (C, D) XAC-X-BY630 (30 nM), (E, F) XAC-X-Texas Red (30 nM) and (G) XAC-dansyl (10 nM). In D and F, images were collected immediately after the fluorescent ligand had been removed from the cells. H shows a representative image of autofluorescence collected under identical conditions to image (G), but without the addition of XAC-dansyl. ADOEA, N⁶-(8-amino-3,6-dioxaoctyl)-5′-ethylamino-5′-oxo-5′-deoxyadenosine; APrEA, N⁶-(3-aminopropyl)-5′-ethylamino-5′-oxo-5′-deoxyadenosine; CHO, Chinese hamster ovary; X, 6-aminohexanoyl; XAC, xanthine amine congener.