Table 6.

Antagonism of fluorescent agonist G_i-mediated responses by DPCPX, XAC and CGS15943

Agonist	log KDDPCPX	n	tog KDXAC	n	log KDCGS	n
NECA	−9.13 ± 0.10	7	−7.86 ± 0.05	14	−8.80 ± 0.10	3
APrEA-X-BY630	−8.70 ± 0.09	8	−7.62 ± 0.07	7	−8.58 ± 0.12	7
ABEA-X-BY630	−8.69 ± 0.06	8	−7.55 ± 0.08	8	−8.54 ± 0.09	8
APEA-X-BY630	−8.61 ± 0.06	8	−7.56 ± 0.08	8	−8.57 ± 0.10	8
AOEA-X-BY630	−8.97 ± 0.07	3	−7.87 ± 0.09	3	−8.87 ± 0.15	3
ADOEA-X-BY630	−8.67 ± 0.09	6	−7.71 ± 0.07	6	−8.67 ± 0.08	6
ABEA-dansyl	−8.76 ± 0.06	6	−7.90 ± 0.06	6	−8.78 ± 0.07	6
ABEA-AO-dansyl	−8.78 ± 0.15	5	−7.74 ± 0.12	5	−8.90 ± 0.19	6
AUEA-dansyl	−8.80 ± 0.12	7	−7.97 ± 0.10	6	−8.96 ± 0.11	7
ABEA-X-BYFL	−8.73 ± 0.16	5	−7.83 ± 0.13	5	−8.69 ± 0.15	6

Values represent mean ± SEM from n separate experiments.

ABEA, N⁶-(4-aminobutyl)-5′-ethylamino-5′-oxo-5′-deoxyadenosine; ADOEA, N⁶-(8-amino-3,6-dioxaoctyl)-5′-ethylamino-5′-oxo-5′-deoxyadenosine; AO, 8-aminooctanoyl; AOEA, N⁶-(8-aminooctyl)-5′-ethylamino-5′-oxo-5′-deoxyadenosine; APEA, N⁶-(5-aminopentyl)- 5′-ethylamino-5′-oxo-5′-deoxyadenosine; APrEA, N⁶-(3-aminopropyl)-5′-ethylamino-5′-oxo-5′-deoxyadenosine; AUEA, N⁶-(11-aminoundecyl)-5′-ethylamino-5′-oxo-5′-deoxyadenosine; CGS 15943, 5-amino-9-chloro-2-(2-furyl)1,2,4-trizolo[1,5-c]quinazoline; DPCPX, 8-cyclopentyl-1,3-dipropylxanthine; NECA, 5′(N-ethylcarboxamido)adenosine; X, 6-aminohexanoyl; XAC, xanthine amine congener.