Figure 7.

Effect of deoxyelephantopin (DET) on the survival rate and pulmonary foci formation in lung metastasis of TS/A mammary tumour cells in female BALB/c mice. Experimental design and details are in Figure S1C. The survival rate curve of test mice in the 10 experimental groups was determined at end points for lung tumour burden (*P < 0.005 and **P < 0.0001) in (A1) and (A2). The excised left lungs of test mice in the 10 experimental groups (top panels). In (B1) and (B2), the number of metastatic pulmonary foci (mean ± SEM) in the left lung was calculated (bottom panels). Values with different letter symbols are significantly different from each other (P < 0.05; one-way anova). (C1) and (C2), representative lung sections of mice from each group stained with haemotoxylin and eosin (100× original magnification). The typical tissue architecture of alveoli can be seen in sham control mice and a fully grown tumour mass in the tumour control group. Immunohistochemical analysis of the level of vascular endothelial growth factor in (D1) and cyclooxygenase-2 in (D2) protein (100× original magnification) in lung tissues of sham control, tumour control and DET- or paclitaxel-treated mice.