Figure 7.

Changes in intracellular Ca²⁺ concentration in B6 treated cells. After treatment with B6 for 24 h, cells were collected and incubated at 37°C with Fluo-3. The intracellular Ca²⁺ concentration was measured by flow cytometry. CICCP was used as a positive control to validate the measurement of intracellular Ca²⁺ release. Results represent the mean ± SD, n= 3. *P < 0.05 versus control. B6, N-(4, 5-dibromo-pyrrole-2-carbonyl)-L-amino isovaleric acid methyl ester; CICCP, carbonyl cyanide-m-chlorophenylhydrazone.