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. 2010 Jan 25;159(4):970–981. doi: 10.1111/j.1476-5381.2009.00605.x

Figure 6.

Figure 6

The effects of tetrandrine on MAPKs signalling in response to hypertrophic stimuli. (A) Time course of phosphorylated and total ERK1/2, p38, JNK1/2, and the effects of tetrandrine (10 µM) on them, in cardiomyocytes treated with Ang II (n= 4). (B) Representative blots of ERK1/2, p38 and JNK1/2 phosphorylation and their total protein expression in the indicated groups of mice (n= 4). (C) The effect of NAC on ERK1/2 activation induced by Ang II in cultured myocytes. Cardiac myocytes were pretreated with 10 mM NAC for 30 min and incubated with Ang II for 120 min. (D) Luciferase assay of the effects of U0126 and NAC on the activities of NF-κB and NFAT (n= 6). Cells were incubated with 1 µM Ang II for up to 48 h. The luciferase assay was performed as described in the Methods. The results were reproducible in three separate experiments. *P < 0.05 versus corresponding control. Ang II, angiogensin II; GAPDH, glyceraldehydes-3-phosphate dehydrogenase; MAPK, mitogen-activated protein kinase; NAC, N-acetylcysteine; NFAT, nuclear factor of activated T cells; NF-κB, nuclear factor-κB.