Table 2.
Results of sdAb competition assay. The biotinylated tracer sdAb was combined with a tenfold molar excess of unbiotinylated inhibitor sdAb. The mean average of duplicate mean fluorescent intensities for each reading relative to no inhibitor was used to calculate the percentage inhibition.
| Percent inhibition | |||||||
|---|---|---|---|---|---|---|---|
| Tracer | |||||||
| A4 | C9 | E7 | G10 | H4 | H8 | ||
| inhibitor | A4 | 98 | 18 | -86 | 0 | 88 | 79 |
| C9 | 50 | 98 | 99 | 86 | 95 | 99 | |
| E7 | 38 | 52 | 84 | 60 | 78 | 95 | |
| G10 | 45 | 36 | -15 | 94 | 92 | 90 | |
| H4 | 70 | 27 | -12 | 85 | 99 | 86 | |
| H8 | 12 | 15 | 65 | 52 | 62 | 91 | |
| BSA | 4 | 4 | -9 | -4 | 0 | -1 | |