Table 1. Reciprocal embryo transfer in mice receiving either DKK1 ADV or empty vectors.
Treatment |
No. of blastocysts transferred |
No. of recipients |
No. of mice with IS (%) |
No. of IS (%) |
No. of blastocysts recovered from mice without IS (%) |
|
---|---|---|---|---|---|---|
Embryo donors | Recipients | |||||
No treatment | Empty ADV | 104 | 7 | 7 (100%) | 60 (58%) | N/A |
No treatment | DKK1 ADV | 135 | 9 | 9 (100%) | 73 (54%) | N/A |
Empty ADV | No treatment | 82 | 6 | 6 (100%) | 46 (56%) | N/A |
DKK1 ADV | No treatment | 98 | 8 | 1 (13%)* | 5 (5%)* | 28 (29%) |
Normal day 4 blastocysts retrieved from untreated mice were transferred into day 4 pseudopregnant recipients receiving treatments of either DKK1 ADV or empty vectors. Conversely, blastocysts retrieved from pregnant females receiving either DKK1 ADV or empty vectors were transferred into the uterine horn of normal untreated pseudopregnant recipients on day 4 midmorning. Implantation was examined 24 hours later by blue dye method. N/A, not applicable.
P<0.05 (Student t-test).