Table 1.
Yields of Recombinant Adeno-associated Virus and Other Recombinant Products, Using Transient Transfection of HEK-293 Cells
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Yield |
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|---|---|---|---|---|---|---|
| Product | Transfection reagent | Culture format | mg/liter | VG/liter | pg/cell (posttransfection) | Ref. |
| IgG | CaPi | Suspension | 5 | — | 2.5 (10 days) | Girard et al. (2002) |
| SEAP | PEI | Suspension | 20 | — | 10 (4 days) | Durocher et al. (2002) |
| SEAP | Liposome | Suspension, SF | 15–20 | — | 10 (4 days) | Schlaeger et al. (2003) |
| Various | CaPi | Suspension | 2.5–28 | — | 1.3–14 (5–7 days) | Baldi et al. (2005) |
| recombinant proteins | PEI | Suspension, SF | 2.6–27 | — | 1.3–14 (6–7 days) | |
| IgG | Liposome | Suspension | 50–80 | — | 25 (4 days) | Liu et al. (2008) |
| IgG | PEI | Suspension | 300 | — | 150 (10 days) | Backliwal et al. (2008b) |
| rAAV | CaPi | Adherent | — | — | ∼0.09 (2 days) | Drittani et al. (2001) |
| PEI | Adherent | — | — | 0.09 (2 days) | ||
| rAAV | PEI | Suspension | — | 5 × 1012 | ∼0.02 (2–3 days) | Park et al. (2006) |
| rAAV | PEI | Suspension, SF | — | 2.6 × 1013 | ∼0.1 (3–4 days) | Durocher et al. (2007) |
| rAAV | CaPi | Adherent | — | 9 × 1013 | 0.8 (4 days) | Hauck et al. (2007) |
| rAAV | CaPi | Adherent | — | 0.5–1.9 × 1013 | 0.05–0.17 (2 days) | Hildinger et al. (2007) |
| PEI | Suspension, SF | — | 0.3–0.6 × 1013 | 0.02–0.07 (2 days) | ||
Abbreviations: CaPi, calcium phosphate; PEI, polyethylenimine; rAAV, recombinant adeno-associated virus; SEAP, secreted human placental alkaline phosphatase; SF, serum free.