Table 2.
Biodistribution of Vector Genomes and Adeno-associated Viral Capsid Sequences in Nonhuman Primate Tissues as Measured by Real-Time Polymerase Chain Reactiona
| |
Animal no. |
||||||||
|---|---|---|---|---|---|---|---|---|---|
| |
24466 |
24527 |
24441 |
24456 |
24499 |
24462 |
24461 |
24479 |
24481 |
| Tissue | (Vector/Cap) | (Vector/Cap) | (Vector/Cap) | (Vector/Cap) | (Vector/Cap) | (Vector/Cap) | (Vector/Cap) | (Vector/Cap) | (Vector/Cap) |
| Gonadal tissue | 0.6/0 | 0.82/0 | 0.41/0 | 1.3/0 | 0.42/0 | 3.3/0 | 0.63/0 | 0.31/0 | 0.76/0 |
| Blood | 0.95/0.03 | 0.59/0 | 1.5/0 | 7.1/0 | 0.2/0 | 1.1/0 | 0.82/0 | 0.54/0 | 1.7/0 |
| Bone marrow | 47/0 | 1.3/0 | 23/0.06 | 35/0.05 | 10/0 | 57/0.08 | 8.1/0 | 4.3/0 | 41/0 |
| Brain | 0.68/0 | 0.02/0 | 0.32/0 | 4.4/0.02 | 0.3/0 | 1.8/0 | 0.97/0 | 1.2/0 | 4.7/0 |
| Lung, left | 180/0 | 1.8/0 | 6.3/0 | 44/0.06 | 8.3/0 | 71/0.06 | 10/0 | 5/0 | 16/0 |
| Lung, right | 34/0 | 0.43/0 | 9.4/0.03 | 13/0.03 | 6.5/0 | 71/0.09 | 7.2/0 | 6/0 | 8/0 |
| Heart | 5.4/0.03 | 0.22/0 | 2.7/0 | 41/0.18 | 2.4/0 | 5.4/0 | 5.6/0 | 3.9/0 | 6.9/0 |
| Skeletal muscle | 6/0 | 0.06/0 | 0.61/0 | 4.3/0 | 0.19/0 | 5.8/0 | 1.5/0 | 0.79/0 | 1.1/0 |
| Stomach | 3.7/0 | 2.9/0 | 8.8/0 | 12/0.03 | 33/0 | 5.4/0 | 5.2/0 | 0.87/0 | 13/0 |
| Duodenum | 24/0 | 4.9/0 | 1.5/0 | 16/0 | 26/0.05 | 20/0 | 14/0 | 14/0.1 | 10/0 |
| Colon | 20/0 | 2.6/0 | 4.6/0 | 6.1/0 | 2/0 | 8.8/0 | 2.2/0 | 6.4/1.1 | 7/0 |
| Pancreas | 11/0.06 | 0.08/0 | 2/0 | 13/0.07 | 100/0.02 | 12/0 | 0.71/0.04 | 0.71/0 | 3.7/0 |
| Gall bladder | 14/0 | 54/0 | 2.7/0 | 46/0 | 240/0 | 890/0 | 0.86/0 | 14/0 | 300/0 |
| Kidney, left | 14/0 | 0.95/0 | 5.7/0.04 | 20/0.12 | 79/0 | 40/0.09 | 6/0 | 6.7/0 | 22/0.02 |
| Kidney, right | 15/0 | 0.58/0 | 5.1/0.03 | 50/0.16 | 81/0.03 | 48/0.08 | 4.6/0 | 13/0 | 12/0 |
| Liver, left | 4,500/1.6 | 140/0.04 | 1,800/2.8 | 14,000/24 | 1,600/0.86 | 5,200/4.8 | 310/0.23 | 590/0.04 | 4,300/2.6 |
| Liver, right | 4,800/1.6 | 160/0.08 | 2,300/3.7 | 10,000/34 | 2,600/0.75 | 5,300/5.1 | 270/0.16 | 750/0.07 | 4,600/2.1 |
| Liver, middle | 5,000/2.5 | 280/0.09 | 1,900/3 | 14,000/15 | 2,400/0.73 | 7,400/5.3 | 320/0.21 | 710/0.07 | 4,300/2.2 |
| Liver, caudate | 4,100/3.7 | 280/0.055 | 1,500/2.7 | 8,500/25 | 1,900/0.9 | 6,500/7 | 320/0.17 | 640/0.05 | 4,400/2.3 |
| Spleen | 3,800/10 | 1,600/1.3 | 1,000/3.4 | 1,500/6.9 | 2,500/2.5 | 1,100/3.2 | 180/0.51 | 490/1.3 | 860/2.2 |
| Mesenteric lymph node | ND/ND | 840/49 | 83/0.33 | 1,100/3.8 | 350/0.64 | 370/0.78 | 130/0 | 250/0.33 | 550/4.2 |
Abbreviation: ND, not determined.
a
The primer–probe sets and PCR conditions are described in Materials and Methods. Data are presented as genome copies of either rAAV.CBEGFP (“Vector”) or wild-type AAV cap (“Cap”) gene per nanogram of total cellular DNA. The tissue source of DNA for analysis is described in the first column.