FIG. 6.

Effect of PEGylation on anticancer efficacy of systemically administered replication-competent adenovirus in the Hep3B tumor model. (a) Effect of vector modification on tumor growth. nu/nu mice with established Hep3B tumors (average volume, 545 mm³) were injected intravenously with a single dose 5 × 10¹⁰ VG of Ad-GL or PEGylated Ad-GL in 100 mm³ of PBS. +PW, mice were pretreated by predosing (intravenous, 3 × 10¹⁰ VG of replication-deficient Ad-DsRed in 100 μl of PBS 4 hr before Ad-GL injection) and administration of warfarin (subcutaneous, 133 μg of warfarin in 100 μl of peanut oil 3 and 1 days before Ad-GL injection). Tumor volume (n = 10 per group) was calculated from tumor dimensions and individual tumor size was plotted. (b) Effect of vector modification on survival. Mice were euthanized when tumor volume reached 2000 mm³ (uncensored event) and Kaplan–Meier survival curves were plotted. +PW, mice were pretreated by predosing and administration of warfarin.