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CSC uses a multistep tandem affinity labeling strategy to confer the desired specificity for the glycoproteins on the cell surface. These steps involve (1) tagging reactive groups from plasma-membrane proteins (yellow triangles, glycans; black, bi-functional linker molecules), (2) cell homogenization, (3) protein digestion and affinity purification, (4) peptide release, (5) peptide analysis by LC-MS/MS and (6) peptide or protein identification.
